Accurately determining the origin of water contamination

Microbial source tracking is a molecular technique to determine the source of faecal bacteria by identifying unique DNA markers in water samples. ADAS performs this test at its molecular biology laboratory in Nottinghamshire.

Arrange a test
Dr Ben Maddison

Dr Ben Maddison

Managing Director – ADAS Biotechnology

Identifying the source of faecal pollution in water

Water pollution caused by untreated sewage or agricultural runoff poses a serious risk to public health and the environment. Traditional water quality tests can confirm the presence of bacteria like E. coli, but they cannot identify their source.

Using microbial source tracking, ADAS Biotechnology accurately determines the origin of water contamination – whether it is more likely to be human or from an animal species.

Microbial source tracking could be useful for water companies, environmental consultants, local authorities, regulators or land managers who need to identify an unknown pollution source quickly.

Female writing on bottle of water sample

How can microbial source tracking be used?

Help focus resources to address the source of contamination.

Guide restoration of and investment in environmentally sensitive sites

Inform infrastructure and land management planning

For bathing water quality assessments

Demonstrate compliance with Water Framework Directives

How can microbial source tracking identify the source of water pollution?

At our molecular biology laboratory in Nottinghamshire, we use a DNA amplification technique known as quantitative polymerase chain reaction (qPCR) to detect DNA markers within a water sample.

These DNA markers are unique to a specific species or group of animals. For example, the human-specific marker HF183. Elevated levels of HF183 in a water sample could indicate sewage discharge is entering the waterway. These molecular tools are so sensitive and precise that they make it possible to predict the source of a pollution incident in any aquatic environment e.g., rivers, lakes or coastlines.

Collecting a water sample for testing

To identify the source of high bacterial counts, ADAS will send you single-use sampling kits with instructions on how to collect a water sample ready for analysis.

When you have received your kits, we will need you to:

  1. collect a representative water sample
  2. add the preservative included with the kit
  3. post the sample back to our lab.

Receive results in as little as 48 hours for urgent cases. Our standard turnaround time is 10 working days.

Arrange a test
Team member in life jacket conducting a water sampling

Beyond a diagnosis

If you require more than a diagnosis, e.g., a site survey, studies or remediation advice, we collaborate with our sister RSK company WRc to deliver a full end-to-end service. To help landowners manage and mitigate identified water quality risks, ADAS farming advisors can provide practical guidance and advice about nutrient management and farm infrastructure.

Project involvement

Helping farmers to improve catchment water quality

Helping farmers to improve catchment water quality

Using microbial source tracking to improve water quality in Brighton and Hove

Using microbial source tracking to improve water quality in Brighton and Hove

Identifying sources of pollution in the River Ray

Identifying sources of pollution in the River Ray